01045pamaa2200265 4500 0000045042 20140919090000.0 090331 eng 9780521521055 (pbk.) QH442.2 HOW 2007 Howe Gene cloning and manipulation Christopher Howe. 2nd ed. Cambridge, UK ; New York: Cambridge University Press, 2007. 266 p.: ill.; 25 cm. CALUK DSCAU Includes bibliographical references (p. [250]-256) and index The tools for the job -- Polymerase chain reaction -- Simple cloning -- Other vector systems for E. coli -- Making libraries -- Screening libraries -- Modification and mutagenesis -- Use of cloned DNA -- Using other organisms Molecular cloning Cloning, Molecular -- methods Escherichia coli -- genetics Gene Library Transformation, Genetic http://www.cambridge.org